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KMID : 0895820040140030079
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Journal of Oriental Rehabilitation Medicine
2004 Volume.14 No. 3 p.79 ~ p.101
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Anti-pathogenetic and Curative Effects of Taeganghwal-tang (Daqianghuo-tang) on the Collagen-Induced Arthritis in Wistar Rats
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Jeong Su-Hyeon
Seo Il-Bok
Kim Soon-Joong
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Abstract
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Objectives: This study was carried out to investigate the anti-pathogenetic and curative effects of Taeganghwal-tang(Daqianghuo-tang) on collagen-induced arthritis in Wistar rats.
Methods: Experiment Ⅰ; Thirty male rats were divided into non-treated normal group, porcine type II collagen immunized control group, and collagen immunized and treated group medicated with extract of Taeganghwal-tang(Daqianghuo-tang) for 15days after collagen immunization. Body weight, paw edema volume, and thickness of ankle joint were measured at 0, 10, 15days after immunization. Arthritic score was evaluated at 11, 12, 13, 14, 15days after immunization. At 15days after immunization, the incidence of arthritis was evaluated, and serum TNF-α, IL-1β and anti-collagen IgG level were analysed, and histopathological examination was performed on the ankle joint.
Experiment Ⅱ; Thirteen rats, that were shown the gross lesions of collagen-induced arthritis, were divided into control group and treated group medicated with extract of Taeganghwal-tang(Daqianghuo-tang) for 15days after onset of arthritis. Non immunized seven rats were served as normal group. Body weight, paw edema volume, and thickness of ankle joint were measured at 0, 5, 10, 15days after treatment. At 15days after treatment, serum TNF-α, IL-1β and anti-collagen IgG level were analysed, and histopathological examination was performed on the ankle joint.
Results: Experiment Ⅰ;
－. Incidence of arthritis was 60% in control group and 10% in treated group.
－. Arthritic score of treated group(0.3±0.9 at 13days, 0.3±0.9 at 14days, 0.3±0.9 at 15days) was significantly decreased(P＜0.01) compared with control group(3.0±2.7 at 13days, 3.61±3.1 at 14days, 3.9±3.4 at 15days) respectively.
－. Paw edema volume(㎖) of treated group(1.90±0.11) was significantly decreased(P＜0.01) compared with control group(2.55±0.53) at 15days after immunization.
－. Thickness of ankle joint(㎜) of treated group(6.64±0.15) was significantly decreased(P＜0.01) compared with control
group(8.35±1.75) at 15days after immunization.
－. Serum TNF-α level(pg/㎖) of treated group(46.00±25.90) was significantly decreased(P＜0.05) compared with control
group(97.52±49.97) at 15days after immunization.
－. Serum IL-1β level(pg/㎖) of treated group(39.40±10.42) was significantly decreased(P＜0.05) compared with control
group(52.22±15.32) at 15days after immunization.
－. Serum anti-collagen IgG level(㎎/㎖) of treated group(0.27±0.16) was significantly decreased(P＜0.05) compared with control group(0.47±0.20) at 15days after immunization.
Experiment Ⅱ;
－. Paw edema volume(㎖) of treated group(3.12±0.28 at 10days, 3.12±0.27 at 15days) was significantly decreased(P＜0.05) compared with control group( 3.72±0.49 at 10days, 3.62±0.48 at 15days) respectively.
－. Serum TNF-α level(pg/㎖) of treated group(84.64±22.04) was significantly decreased(P＜0.05) compared with control
group(135.31±55.56) at 15days after treatment.
－. Serum IL-1β level(pg/㎖) of treated group(58.16±7.28) was not significantly different compared with control group(66.75±10.68) at 15days after treatment.
－. Serum anti-collagen IgG level(㎎/㎖) of treated group(0.78±0.09) was not significantly different compared with control
group(0.87±0.08) at 15days after treatment.
－. Histopathologically, destructive lesions of articular cartilage and subchondral bony tissue, and degree of fibrous ankylosis in treated group were alleviated compared with those of control group at 15 days after treatment.
Conclusions: These results indicated that Taeganghwal-tang(Daqianghuo-tang) has the anti-pathogenetic effects on the onset of collagen-induced arthritis in rats, by it’s inhibitory effects on the production of anti-collagen IgG. And also, Taeganghwal-tang(Daqianghuo-tang) has inhibitory effects on the progression of collagen-induced arthritis in rats, by it’s lowering effects on the secretion of TNF-α.
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KEYWORD
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Taeganghwal-tang(Daqianghuo-tang)(Daqianghuo-tang) collagen-induced arthritis, TNF-α, IL-1β, anti-collagen IgG
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